Ride organizers say more than 3,000 volunteers will help the riders make their way to La Grange for an overnight stop and then on to Austin on Sunday.
Kelli Dreiling is with the National MS Society and says riders should remember a few important things.
"Make sure that you've got your rider numbers in place, your water bottles filled, have your map in your back pocket of your jersey. Make sure if you're camping out that you've got adequate sleepwear because it will get chilly. It's going to be in the 50's. Be prepared and have fun. It's not a race. I know a lot of people refer to it as a race, but it's not, it's just a ride. So take your time and enjoy it and have fun."
There are three start points for the ride: Tully Stadium in West Houston, Rhodes Stadium in Katy and Waller High School in Waller.
Dreiling says Mother Nature might make things a bit difficult on the first leg of the ride.
"The wind is not looking so favorable. It's looking like it's going to be coming from the north right into the rider's faces and at a crosswind, so I know they're not going to be happy about that. But there's a silver lining because on Sunday it's supposed to be much better with a west wind, so it will be a fantastic finish into Austin."
Dreiling says rest areas will be set up every 6-12 miles along the course. Riders are hoping to raise $17 million for MS research.